New PDF release: Automated DNA Sequencing and Analysis

By Mark D. Adams, Chris Fields, J. Craig Venter

ISBN-10: 0080926398

ISBN-13: 9780080926391

ISBN-10: 0127170103

ISBN-13: 9780127170107

A well timed publication for DNA researchers, computerized DNA Sequencing and research stories and assesses the cutting-edge of automatic DNA series analysis-from the development of clone libraries to the developmentof laboratory and group databases. It offers the methodologies and methods of automatic DNA series research in a manner that enables them to be in comparison and contrasted. through taking a vast view of the Read more...

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Additional resources for Automated DNA Sequencing and Analysis

Example text

With 3000 6-mers and 7-mers, every base is going to be read on averag e four times, but positively scored prob es can not be correctly ordered . The missing inform ation requi res tens of thousand s of additional probings (Drmanac et al. , 1989). On the other hand , the limiting factors for largescale seque ncing by gels are the short length of accurate reads and the need for redundant sequencing, especially in the shotgun approac h. Fortun atel y, it is very simple to produce data with a 5-10% error rate by long gel reads of minimally overlapped clones selected on the basis of hybridization data .

Surzyski , personal communication ). Th ese meth ods can be grouped into th ree categories: direct ed clonin g (Sa nger et al. , 1980; Banki er et al. , 1987; Phadnis et al. , 1990a; Strat hmann et al. , 1991; Fitzgera ld et al. , 1992; S. Surzyski, persona l comm unicatio n) . In the directed cloning approach, fragme nts of the DNA of intere st are digested with a se ries of restriction endonucleases and cloned directly into M13, pUC or oth er vectors with the appropriate unique cloning sites and selection methods.

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Automated DNA Sequencing and Analysis by Mark D. Adams, Chris Fields, J. Craig Venter


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